Pengujian Toksisitas Kulit Batang Mitragyna speciosa

  • Agmi Sinta Putri Faculty of Forestry, Mulawarman University
  • Harlinda Kuspradini Faculty of Forestry, Mulawarman University
  • Enih Rosamah Faculty of Forestry, Mulawarman University
  • Dian Puspitasari Faculty of Forestry, Mulawarman University
Keywords: Brine Shrimp Lethality Test (BSLT), Mitragyna speciosa, Secondary metabolite, Toxic

Abstract

Indonesia is one of the countries that has mega biodiversity. Several medicinal plants contain bioactive compounds which used to prevent and treat illness. One of the plants that used as medicine by traditional people is Mitragyna speciosa. This study aimed to determine the secondary metabolite compounds and LC50 value from the stem bark of M. speciosa. The sample extracted using three organic solvents is n-hexane, ethyl acetate, and ethanol. Secondary metabolite compounds were analyzed by phytochemical screening based on color change. The LC50 value assayed by Brine Shrimp Lethality Test (BSLT) method using Artemia salina larvae as tested animals. The yield of extraction from the highest to lowest presentation was obtained in ethanol, ethyl acetate, and n-hexane extract, respectively. The secondary metabolite compounds contained in all extracts were variated. The result of the toxicity assay showed that LC50 < 100 ppm caused the 50% mortality of larvae. The third of the extracts are showed toxic effects. N-hexane extract has the highest toxicity at a concentration of 114 ppm, while the lowest toxicity found in ethyl acetate extract at a concentration of 414 ppm. Further study is very needed to support the potential of utilization from M. speciosa as medicinal plants.

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Published
2021-07-10
How to Cite
Putri, A. S., Kuspradini, H., Rosamah , E., & Puspitasari, D. (2021). Pengujian Toksisitas Kulit Batang Mitragyna speciosa . MCTrops , 1(2), 8-13. Retrieved from http://ij.unmul.ac.id/ojs/index.php/nls/article/view/12